.png){kind=icon}
返回
Capturing nucleic acid variants with precision using CRISPR diagnostics
.png){kind=icon}
PRE
AI
DOI：10.1016/j.bios.2022.114712.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
CRISPR/Cas systems have the ability to precisely target nucleotide sequences and enable their rapid identifi-cation and modification. While nucleotide modification has enabled the therapeutic correction of diseases, the process of identifying the target DNA or RNA has greatly expanded the field of molecular diagnostics in recent times. CRISPR-based DNA/RNA detection through programmable nucleic acid binding or cleavage has been demonstrated for a large number of pathogenic and non-pathogenic targets. Combining CRISPR detection with nucleic acid amplification and a terminal signal readout step allowed the development of numerous rapid and robust nucleic acid platforms. Wherever the Cas effector can faithfully distinguish nucleobase variants in the target, the platform can also be extended for sequencing-free rapid variant detection. Some initial PAM disruption-based SNV detection reports were limited to finding or integrating mutated/mismatched nucleotides within the PAM sequences. In this review, we try to summarize the developments made in CRISPR diagnostics (CRISPRDx) to date emphasizing CRISPR-based SNV detection. We also discuss the applications where such diagnostic modalities can be put to use, covering various fields of clinical research, SNV screens, disease gen-otyping, primary surveillance during microbial infections, agriculture, food safety, and industrial biotechnology. The ease of rapid design and implementation of such multiplexable assays can potentially expand the applica-tions of CRISPRDx in the domain of affinity-based target sequencing, with immense possibilities for low-cost, quick, and widespread usage. In the end, in combination with proximity assays and a suicidal gene approach, CRISPR-based in vivo SNV detection and cancer cell targeting can be formulated as personalized gene therapy.
Keyword:
CRISPR
Cas
CRISPRDx
Single nucleotide variants
Mismatch sensitivity
Therapeutic applications
期刊
IF:
10.5
论文数:
1.8W
被引数:
7.7W
机构
引用论文
Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp.png)
SCIENTIFIC REPORTS
OAAI
SCIENTIFIC REPORTSIF3.9
OAAI分享收藏Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing2类CRISPR/Cas: 用于和超越基因组编辑的扩展生物技术工具箱CELL AND BIOSCIENCEOAAI
CELL AND BIOSCIENCEIF6.2
OAAI分享收藏Designing sensitive viral diagnostics with machine learning用机器学习设计敏感的病毒诊断NATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAI分享收藏CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cellsCASFISH: 固定细胞中基因组位点的CRISPR/Cas9-mediated原位标记OAAI
OAAI分享收藏Detection of SARS-CoV-2 and Its Mutated Variants via CRISPR-Cas13-Based Transcription AmplificationANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏
查看更多内容