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Clonal tracking using embedded viral barcoding and high-throughput sequencing
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DOI：10.1038/s41596-019-0290-z.png)
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摘要
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Embedded viral barcoding in combination with high-throughput sequencing is a powerful technology with which to track single-cell clones. It can provide clonal-level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy. Here, we present a detailed protocol for a viral barcoding procedure that includes the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing data. The entire procedure can be completed within a few weeks. This barcoding method requires cells to be susceptible to viral transduction. It provides high sensitivity and throughput, and enables precise quantification of cellular progeny. It is cost efficient and does not require any advanced skills. It can also be easily adapted to many types of applications, including both in vitro and in vivo experiments. This protocol describes the generation and delivery of embedded viral barcode libraries to track single-cell clones. Barcodes are amplified from genomic DNA and quantified by high-throughput sequencing and bioinformatics analysis.
Keyword:
POLYMERASE-CHAIN-REACTION
STEM-CELLS
LINEAGE RELATIONSHIPS
DIFFERENTIATION
DYNAMICS
RECONSTITUTION
EXPRESSION
DIVERSE
GROWTH
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被引数:
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