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Complex Nucleic Acid Hybridization Reactions inside Capillary-Driven Microfluidic Chips
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DOI：10.1002/smll.202005476.png)
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摘要
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Nucleic acid hybridization reactions play an important role in many (bio)chemical fields, for example, for the development of portable point-of-care diagnostics, and often such applications require nucleic acid-based reaction systems that ideally run without enzymes under isothermal conditions. The use of novel capillary-driven microfluidic chips to perform two isothermal nucleic acid hybridization reactions, the simple opening of molecular beacon structures and the complex reaction cascade of a clamped-hybridization chain reaction (C-HCR), is reported here. For this purpose, reagents are arranged in a self-coalescence module (SCM) of a passive silicon microfluidic chip using inkjet spotting. The SCM occupies a footprint of approximate to 7 mm(2) of a approximate to 0.4 x 2 cm(2) microfluidic chip. By means of fluorophore-labeled DNA probes, the hybridization reactions can be analyzed in just approximate to 2 min and using only approximate to 3 mu L of the sample. Furthermore, the SCM chip offers a variety of reagent delivery options, allowing, for example, the influence of the initiator concentration on the kinetics of C-HCR to be investigated systematically with minimal sample and time requirements. These results suggest that self-powered microfluidic chips equipped with a SCM provide a powerful platform for performing and investigating complex reaction systems.
Keyword:
capillary‐ driven chip
clamped‐ hybridization chain reaction
molecular beacon reaction
self‐ coalescence module
signal amplification
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IF:
12.1
论文数:
3.0W
被引数:
16.4W
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