.png){kind=icon}
返回
Comprehensive microcrystal electron diffraction sample preparation for cryo-EM
.png){kind=icon}
PRE
AI
DOI：10.1038/s41596-024-01088-7.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Microcrystal electron diffraction (MicroED) has advanced structural methods across a range of sample types, from small molecules to proteins. This cryogenic electron microscopy (cryo-EM) technique involves the continuous rotation of small 3D crystals in the electron beam, while a high-speed camera captures diffraction data in the form of a movie. The crystal structure is subsequently determined by using established X-ray crystallographic software. MicroED is a technique still under development, and hands-on expertise in sample preparation, data acquisition and processing is not always readily accessible. This comprehensive guide on MicroED sample preparation addresses commonly used methods for various sample categories, including room temperature solid-state small molecules and soluble and membrane protein crystals. Beyond detailing the steps of sample preparation for new users, and because every crystal requires unique growth and sample-preparation conditions, this resource provides instructions and optimization strategies for MicroED sample preparation. The protocol is suitable for users with expertise in biochemistry, crystallography, general cryo-EM and crystallography data processing. MicroED experiments, from sample vitrification to final structure, can take anywhere from one workday to multiple weeks, especially when cryogenic focused ion beam milling is involved.
Keyword:
MEAN FREE-PATH
CRYOELECTRON MICROSCOPY
PROTEIN CRYSTALS
SPECIMEN PREPARATION
ATOMIC-RESOLUTION
RADIATION-DAMAGE
3-DIMENSIONAL STRUCTURE
SCATTERING FACTORS
DATA-COLLECTION
LIVER CATALASE
期刊
IF:
16
论文数:
4.0K
被引数:
5.6W
机构
暂无机构信息
引用论文
Self-modification of titanium dioxide materials by Ti3+ and/or oxygen vacancies: new insights into defect chemistry of metal oxidesTi3 和/或氧空位对二氧化钛材料的自改性: 金属氧化物缺陷化学的新见解.png)
RSC Adv.
RSC Adv.IF0
PREAI
分享收藏
分享收藏分享收藏Structural resolution of a small organic molecule by serial X-ray free-electron laser and electron crystallographyNATURE CHEMISTRY
OAAI
NATURE CHEMISTRYIF20.2
OAAI分享收藏Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellaeOAAI
OAAI分享收藏
查看更多内容