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Confocal multiview light-sheet microscopy

delete2015-11-25
delete81
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OA
AI
G
Gustavo de Medeiros
N
Nils Norlin
S
Stefan Günther
M
Marvin Albert
L
Laura Panavaite
U
Ulla-Maj Fiúza
F
Francesca Peri
T
Takashi Hiiragi
U
Uroš Kržič
L
Lars Hufnagel *
DOI:10.1038/ncomms9881delete
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摘要

摘要

En 中文
Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image contrast. Here we combine multiview light-sheet imaging with electronic confocal slit detection implemented on modern camera sensors. In addition to improved imaging quality, the electronic confocal slit detection doubles the acquisition speed in multiview setups with two opposing illumination directions allowing simultaneous dual-sided illumination. Confocal multiview light-sheet microscopy eliminates the need for specimen-specific data fusion algorithms, streamlines image post-processing, easing data handling and storage.
Keyword:
PLANE ILLUMINATION MICROSCOPY
REPORTER MOUSE
EMBRYOS
DEEP
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期刊

Nature Communications 封面图
Nature Communications
IF:
15.7
论文数:
9.3W
被引数:
91.2W

机构

E
european molecular biology laboratory (embl)
学者数:
8.3K
论文数: 5.1K
被引数: 31
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