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Digital microfluidics for cell-based assays

delete2008-01-01
delete397
PRE
AI
I
Irena Barbulovic-Nad
H
Hao Yang
P
Philip S. Park
A
Aaron R. Wheeler *
DOI：10.1039/b717759cdelete
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摘要

摘要

En 中文
We introduce a new method for implementing cell-based assays. The method is based on digital microfluidics (DMF) which is used to actuate nanolitre droplets of reagents and cells on a planar array of electrodes. We demonstrate that this method is advantageous for cell-based assays because of automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. A cytotoxicity assay using Jurkat T-cells was performed using the new method, which had similar to 20 times higher sensitivity than a conventional well plate assay. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays on the microscale.
Keyword:
ON-A-CHIP
MANIPULATION
DEVICE
DEFORMATION
ADSORPTION
GRADIENTS
PERFUSION
DROPLETS
ARRAYS
AI总结

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

L
Lab on a Chip
IF:
5.4
论文数:
9.0K
被引数:
3.3W

机构

U
university of toronto
学者数:
14.8W
论文数: 12.0W
被引数: 165
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