.png){kind=icon}
返回
DNA/chitosan electrostatic complex
.png){kind=icon}
PRE
AI
DOI：10.1016/j.ijbiomac.2016.03.035.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Up to now, chitosan and DNA have been investigated for gene delivery due to chitosan advantages. It is recognized that chitosan is a biocompatible and biodegradable non-viral vector that does not produce immunological reactions, contrary to viral vectors. Chitosan has also been used and studied for its ability to protect DNA against nuclease degradation and to transfect DNA into several kinds of cells. In this work, high molecular weight DNA is compacted with chitosan. DNA-chitosan complex stoichiometry, net charge, dimensions, conformation and thermal stability are determined and discussed. The influence of external salt and chitosan molecular weight on the stoichiometry is also discussed. The isoelectric point of the complexes was found to be directly related to the protonation degree of chitosan. It is clearly demonstrated that the net charge of DNA-chitosan complex can be expressed in terms of the ratio [NH3+]/[P-], showing that the electrostatic interactions between DNA and chitosan are the main phenomena taking place in the solution. Compaction of DNA long chain complexed with low molar mass chitosan gives nanoparticles with an average radius around 150 nm. Stable nanoparticles are obtained for a partial neutralization of phosphate ionic sites (i.e.: [NH3+]/[P-] fraction between 0.35 and 0.80). (C) 2016 Elsevier B.V. All rights reserved.
Keyword:
DNA
Chitosan
Electrostatic interactions
Compaction
Stability
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
8.5
论文数:
5.0W
被引数:
21.7W
机构
引用论文
Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency
PREAI
分享收藏
分享收藏Mechanistic study of transfection of chitosan/DNA complexes coated by anionic poly(γ-glutamic acid).png)
BIOMATERIALS
BIOMATERIALSIF12.9
PREAI
分享收藏The influence of charge density of chitosan in the compaction of the polyanions DNA and xanthanBIOMACROMOLECULES
BIOMACROMOLECULESIF5.4
PREAI
分享收藏
查看更多内容