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DNAzyme-powered nucleic acid release from solid supports
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DOI：10.1039/c9cc07790a.png)
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摘要
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Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.
Keyword:
RNA
DNA
ENZYME
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4.2
论文数:
5.9W
被引数:
16.5W
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CARCINOGENESISIF2.9
OAAI分享收藏Unimolecular Catalytic DNA Biosensor for Amplified Detection of L-Histidine via an Enzymatic Recycling Cleavage StrategyANALYTICAL CHEMISTRY
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ANALYTICAL CHEMISTRYIF6.7
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