.png){kind=icon}
返回
Does conservation account for splicing patterns?
.png){kind=icon}
OA
AI
DOI：10.1186/s12864-016-3121-4.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Background: Alternative mRNA splicing is critical to proteomic diversity and tissue and species differentiation. Exclusion of cassette exons, also called exon skipping, is the most common type of alternative splicing in mammals. Results: We present a computational model that predicts absolute (though not tissue-differential) percent-spliced-in of cassette exons more accurately than previous models, despite not using any 'hand-crafted' biological features such as motif counts. We achieve nearly identical performance using only the conservation score (mammalian phastCons) of each splice junction normalized by average conservation over 100 bp of the corresponding flanking intron, demonstrating that conservation is an unexpectedly powerful indicator of alternative splicing patterns. Using this method, we provide evidence that intronic splicing regulation occurs predominantly within 100 bp of the alternative splice sites and that conserved elements in this region are, as expected, functioning as splicing regulators. We show that among conserved cassette exons, increased conservation of flanking introns is associated with reduced inclusion. We also propose a new definition of intronic splicing regulatory elements (ISREs) that is independent of conservation, and show that most ISREs do not match known binding sites or splicing factors despite being predictive of percent-spliced-in. Conclusions: These findings suggest that one mechanism for the evolutionary transition from constitutive to alternative splicing is the emergence of cis-acting splicing inhibitors. The association of our ISREs with differences in splicing suggests the existence of novel RNA-binding proteins and/or novel splicing roles for known RNA-binding proteins.
Keyword:
Alternative splicing
Conservation
Splicing regulation
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3.7
论文数:
1.9W
被引数:
5.2W
机构
引用论文
Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity.png)
Gene
GeneIF0
PREAI
分享收藏THE MEANING AND USE OF THE AREA UNDER A RECEIVER OPERATING CHARACTERISTIC (ROC) CURVE受试者工作特征 (ROC) 曲线下面积的含义和用途RADIOLOGY
RADIOLOGYIF15.2
PREAI
分享收藏Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes脊椎动物，昆虫，蠕虫和酵母基因组中的进化保守元素GENOME RESEARCH
OAAI
GENOME RESEARCHIF5.5
OAAI分享收藏Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteinsNATURE BIOTECHNOLOGY
NATURE BIOTECHNOLOGYIF41.7
PREAI
分享收藏分享收藏
查看更多内容