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Effect of dietary resveratrol on slow oxidative muscle fiber expression and energy metabolism in beef muscle via AMPK/SIRT1/PGC-1α signaling pathway
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DOI：10.1016/j.meatsci.2026.110112.png)
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Abstract
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This study aimed to investigate whether dietary resveratrol in vivo promotes the transition of beef muscle fiber types from type IIx to I by regulating the adenosine monophosphate activated protein kinase (AMPK)/sirtuin1 (SIRT1)/peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) signaling pathway and influences post-mortem energy metabolism processes. Twelve cattle were randomly divided into two groups in this study: the control group (CON, basal diet) and resveratrol supplementation group (RES, basal diet containing resveratrol (5 g/animal/day)). After 120 days of feeding, the cattle were slaughtered for analysis. Through analysis of key gene and protein expression levels, this study confirmed that resveratrol significantly activated the AMPK/SIRT1/PGC-1α pathway, promoted the expression of PGC-1α downstream mitochondrial biogenesis-related factors (myocyte enhancer factor 2C, nuclear respiratory factor 1, nuclear respiratory factor 2 and mitochondrial transcription factor A), thereby inducing a phenotypic transition from type IIx to I fibers. This promotive effect of resveratrol exhibits muscle-dependent specificity for certain genes and proteins, where the expression of AMPK/SIRT1/PGC-1α was higher in the psoas major (PM) compared to the longissimus lumborum (LL) and semitendinosus (ST). Dietary resveratrol improved the activity of the TCA cycle and creatine kinase enzyme, while reducing glycolytic enzymes activity except phosphofructokinase. It significantly reduced glycogen and lactate content in the LL at 45 min post-mortem, as well as glycogen levels in the PM and ST. After resveratrol treatment, glycolytic potential was significantly decreased in LL and PM, but no significant difference was observed in ST. These results indicated that resveratrol differentially regulated energy metabolism in different muscles.
Keywords:
resveratrol
AMPK/SIRT1/PGC-1α pathway
muscle fiber type transition
energy metabolism
beef muscle
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