.png){kind=icon}
返回
eSGA:: E. coli synthetic genetic array analysis
.png){kind=icon}
PRE
AI
DOI：10.1038/NMETH.1239.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Physical and functional interactions define the molecular organization of the cell. Genetic interactions, or epistasis, tend to occur between gene products involved in parallel pathways or interlinked biological processes. High-throughput experimental systems to examine genetic interactions on a genome-wide scale have been devised for Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans and Drosophila melanogaster, but have not been reported previously for prokaryotes. Here we describe the development of a quantitative screening procedure for monitoring bacterial genetic interactions based on conjugation of Escherichia coli deletion or hypomorphic strains to create double mutants on a genome-wide scale. The patterns of synthetic sickness and synthetic lethality (aggravating genetic interactions) we observed for certain double mutant combinations provided information about functional relationships and redundancy between pathways and enabled us to group bacterial gene products into functional modules.
Keyword:
ESCHERICHIA-COLI
HOMOLOGOUS RECOMBINATION
THIOREDOXIN REDUCTASE
INTERACTION NETWORK
PROTEIN COMPLEXES
YEAST
SYSTEM
OPERON
REQUIREMENT
BIOGENESIS
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
机构
引用论文
.png){kind=icon}
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants:: the Keio collectionK-12框内单基因敲除突变体的大肠杆菌的构建:: Keio collection
OAAI
OAAI分享收藏
查看更多内容