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Fast multicolor 3D imaging using aberration-corrected multifocus microscopy
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DOI：10.1038/NMETH.2277.png)
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Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
Keyword:
POINT-SPREAD FUNCTION
QUANTUM-DOT
DYNAMICS
TRACKING
YEAST
TIME
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
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