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Faster STORM using compressed sensing
DOI:10.1038/NMETH.1978.png)
摘要
En 中文
In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s.
Keyword:
OPTICAL RECONSTRUCTION MICROSCOPY
LOCALIZATION MICROSCOPY
SUPERRESOLUTION MICROSCOPY
INFORMATION
RESOLUTION
DYNAMICS
CELLS
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
机构
引用论文
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