arrow
返回

Faster STORM using compressed sensing

delete2012-04-22
delete450
delete
OA
AI
朱磊 封面图
朱磊 (Lei Zhu) *
张卫 封面图
张卫 (Wei Zhang)
D
Daniel Elnatan
B
Bo Huang
DOI:10.1038/NMETH.1978delete
delete原文链接
delete原文求助
delete分享
delete收藏
摘要

摘要

En 中文
In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s.
Keyword:
OPTICAL RECONSTRUCTION MICROSCOPY
LOCALIZATION MICROSCOPY
SUPERRESOLUTION MICROSCOPY
INFORMATION
RESOLUTION
DYNAMICS
CELLS

期刊

Nature Methods 封面图
Nature Methods
IF:
32.1
论文数:
7.2K
被引数:
12.7W

机构

G
Georgia Institute of Technology
学者数:
1.8W
论文数: 1.4W
被引数: 5.9W
U
university system of georgia
学者数:
7.3W
论文数: 6.5W
被引数: 101
引用论文

引用论文

Microscopy and its focal switch
err2008-12-17
err876
errOAAI
errHell, Stefan W.
err分享
err收藏
High-density localization of active molecules using Structured Sparse Model and Bayesian Information Criterion
err2011-08-15
err79
errOAAI
errQuan, Tingwei; Zhu, Hongyu; Liu, Xiaomao; Liu, Yongfeng; Ding, Jiuping; Zeng, Shaoqun; Huang, Zhen-Li
err分享
err收藏
Current indications for open stone surgery in an endourology center
err1995-02-01
err0
PREAI
errChristopher J. Kane; Damien M. Bolton; Marshall L. Stoller
err分享
err收藏
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics
err2008-04-13
err763
errOAAI
errShroff, Hari; Galbraith, Catherine G.; Galbraith, James A.; Betzig, Eric
err分享
err收藏
Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution
err2008-11-23
err569
errOAAI
errHuang, Bo; Jones, Sara A.; Brandenburg, Boerries; Zhuang, Xiaowei
err分享
err收藏
Fast, three-dimensional super-resolution imaging of live cells活细胞的快速三维超分辨率成像
err2011-05-08
err754
errOAAI
errJones, Sara A.; Shim, Sang-Hee; He, Jiang; Zhuang, Xiaowei
err分享
err收藏
学者 查看更多内容