.png){kind=icon}
返回
Fluorophore localization algorithms for super-resolution microscopy
.png){kind=icon}
PRE
AI
DOI：10.1038/NMETH.2844.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Super-resolution localization microscopy methods provide powerful new capabilities for probing biology at the nanometer scale via fluorescence. These methods rely on two key innovations: switchable fluorophores (which blink on and off and can be sequentially imaged) and powerful localization algorithms (which estimate the positions of the fluorophores in the images). These techniques have spurred a flurry of innovation in algorithm development over the last several years. In this Review, we survey the fundamental issues for single-fluorophore fitting routines, localization algorithms based on principles other than fitting, three-dimensional imaging, dipole imaging and techniques for estimating fluorophore positions from images of multiple activated fluorophores. We offer practical advice for users and adopters of algorithms, and we identify areas for further development.
Keyword:
SINGLE-MOLECULE LOCALIZATION
POINT-SPREAD FUNCTION
OPTICAL RECONSTRUCTION MICROSCOPY
MULTIFOCAL PLANE MICROSCOPY
HIGH-DENSITY LOCALIZATION
FLUORESCENCE MICROSCOPY
PARTICLE-TRACKING
SCMOS CAMERA
ORIENTATION ESTIMATION
DIFFRACTION-LIMIT
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
机构
引用论文
Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution非线性结构照明显微镜: 理论上无限分辨率的宽场荧光成像
OAAI
OAAI分享收藏Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.png)
NATURE METHODS
NATURE METHODSIF32.1
PREAI
分享收藏Fast Fourier domain localization algorithm of a single molecule with nanometer precisionOPTICS LETTERS
OPTICS LETTERSIF3.3
PREAI
分享收藏Localization-based super-resolution microscopy with an sCMOS camera part III: camera embedded data processing significantly reduces the challenges of massive data handlingOPTICS LETTERS
OPTICS LETTERSIF3.3
PREAI
分享收藏Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)通过随机光学重建显微镜 (STORM) 进行亚衍射极限成像NATURE METHODS
OAAI
NATURE METHODSIF32.1
OAAI分享收藏
查看更多内容