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FoldAffinity: binding affinities from nDSF experiments
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DOI：10.1038/s41598-021-88985-z.png)
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摘要
En 中文
Differential scanning fluorimetry (DSF) using the inherent fluorescence of proteins (nDSF) is a popular technique to evaluate thermal protein stability in different conditions (e.g. buffer, pH). In many cases, ligand binding increases thermal stability of a protein and often this can be detected as a clear shift in nDSF experiments. Here, we evaluate binding affinity quantification based on thermal shifts. We present four protein systems with different binding affinity ligands, ranging from nM to high mu M. Our study suggests that binding affinities determined by isothermal analysis are in better agreement with those from established biophysical techniques (ITC and MST) compared to apparent K(d)s obtained from melting temperatures. In addition, we describe a method to optionally fit the heat capacity change upon unfolding (Delta Cp) during the isothermal analysis. This publication includes the release of a web server for easy and accessible application of isothermal analysis to nDSF data.
Keyword:
MULTIPLE SEQUENCE ALIGNMENT
PROTEIN
STABILITY
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期刊
IF:
3.9
论文数:
28.0W
被引数:
83.5W
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