.png){kind=icon}
返回
High-throughput multiplexed fluorescence-activated droplet sorting
.png){kind=icon}
OA
AI
DOI：10.1038/s41378-018-0033-2.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Fluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous populations. Our experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.
Keyword:
PROTEIN-SIGNALING NETWORKS
MAMMALIAN-CELLS
MICROFLUIDICS
MANIPULATION
SELECTION
BREAKUP
ASSAYS
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
M
IF:
9.9
论文数:
1.3K
被引数:
6.7K
机构
引用论文
Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption基于细胞外代谢物产生或消耗的单细胞微流控高通量培养选择.png)
NATURE BIOTECHNOLOGY
OAAI
NATURE BIOTECHNOLOGYIF41.7
OAAI分享收藏Simultaneous measurement of multiple active kinase states using polychromatic flow cytometryNATURE BIOTECHNOLOGY
NATURE BIOTECHNOLOGYIF41.7
PREAI
分享收藏分享收藏分享收藏Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS)通过吸光度激活液滴分选 (AADS) 进行超高通量定向酶进化OAAI
OAAI分享收藏CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidicsANALYSTOAAI
ANALYSTIF3.3
OAAI分享收藏
查看更多内容