.png){kind=icon}
返回
Homogeneous assays using aptamers
.png){kind=icon}
PRE
AI
DOI：10.1039/c0an00281j.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Aptamers are DNA or RNA oligonucleotides that can bind with high affinity and specificity to a wide range of targets such as proteins, metal ions or pathogenic microorganisms. Soluble aptamers and aptazymes have been used as sensing elements for developing homogeneous assays in a solution phase, the whole sensing process being carried out in a homogeneous solution. Contrary to most conventional heterogeneous assays that are time-consuming and labor-intensive, aptamer-based homogeneous assays are simple, easy-toperform, rapid and do not require immobilization nor washing steps. To our knowledge, this review is the first entirely dedicated to aptamer-based homogeneous assays. Optical detection appears as the most developed technique. Colorimetry represents the simplest sensing mode that occupies a very important position among aptamer-based assays, involving gold nanoparticle aggregation (with unmodified or aptamer-modified gold NPs), the formation of HRP-mimicking DNAzyme with hemin, dye displacement or interactions with a cationic polymer. Fluorescence that is highly sensitive offers the most developed detection mode. Aptamers can be labeled or not, to give rise to turn-on or usually less sensitive turn-off fluorescent assays. Newly reported and thus less developed non-conventional magnetic resonance imaging (MRI) and electrochemistry also recently appeared in the literature, thrombin still remains the main detected target. Homogeneous assays based on aptazyme, an aptamer sequence connected to a known ribozyme motif, are also described in this review, involving optical detection, by colorimetry or fluorescence.
Keyword:
IN-VITRO SELECTION
FUNCTIONALIZED GOLD NANOPARTICLES
HIGHLY SENSITIVE DETECTION
RESONANCE ENERGY-TRANSFER
NUCLEIC-ACID
LABEL-FREE
PROTEIN-DETECTION
QUANTUM-DOT
DNA APTAMERS
ELECTROCHEMICAL DETECTION
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
A
IF:
3.3
论文数:
1.6W
被引数:
3.3W
机构
引用论文
CdS nanorod arrays with TiO2 nano-coating for improved photostability and photocatalytic activity
PREAI
分享收藏The seasonal variation of the CO2 flux over Tropical Asia estimated from GOSAT, CONTRAIL, and IASI
OAAI
OAAI分享收藏分享收藏Real-World Treatment Patterns for Lung Neuroendocrine Tumors: A Claims Database Analysis.png)
Oncology
OncologyIF0
PREAI
分享收藏RNA aptamers that bind L-arginine with sub-micromolar dissociation constants and high enantioselectivityNUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAI分享收藏Protein-dependent ribozymes report molecular interactions in real timeNATURE BIOTECHNOLOGY
NATURE BIOTECHNOLOGYIF41.7
PREAI
分享收藏
查看更多内容