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Human L1 retrotransposition:: cis preference versus trans complementation
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DOI：10.1128/MCB.21.4.1429-1439.2001.png)
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摘要
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Long interspersed nuclear elements (LINEs or L1s) comprise approximately 17% of human DNA; however, only about 60 of the similar to 400,000 L1s are mobile, Using a retrotransposition assay in cultured human cells, we demonstrate that. L1-encoded proteins predominantly mobilize the RNA that encodes them. At much lower levels, L1-encoded proteins can act in trails to promote retrotransposition of mutant L1s and other cellular mRNAs, creating processed pseudogenes. Mutant L1 RNAs are mobilized at 0.2 to 0.9% of the retrotransposition frequency of wild-type L1s, whereas cellular RNAs are mobilized at much lower frequencies (ca. 0.01 to 0.05% of wild-type levels). Thus, we conclude that L1-encoded proteins demonstrate a profound cis preference for their encoding RNA. This mechanism could enable L1 to remain retrotransposition competent in the presence of the overwhelming number of nonfunctional L1s present in human DNA.
Keyword:
HIGH-FREQUENCY RETROTRANSPOSITION
HUMAN TRANSPOSABLE ELEMENT
REVERSE TRANSCRIPTION
RIBONUCLEOPROTEIN-PARTICLES
MAMMALIAN-CELLS
REPEAT FAMILY
RNA
ENDONUCLEASE
SEQUENCES
INTEGRATION
期刊
IF:
2.7
论文数:
1.3W
被引数:
3.8W
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分享收藏Determination of L1 retrotransposition kinetics in cultured cells培养细胞中L1逆转录动力学的测定.png)
NUCLEIC ACIDS RESEARCH
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NUCLEIC ACIDS RESEARCHIF13.1
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