.png){kind=icon}
返回
Human RAD50 makes a functional DNA-binding complex
.png){kind=icon}
PRE
AI
DOI：10.1016/j.biochi.2015.03.017.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The MRE11-RAD50-NBS1 (MRN) complex has several distinct functions in DNA repair including important roles in both non-homologous end-joining (NHEJ) and homologous recombination (HR). The biochemical activities of MR(N) have been well characterized implying specific functional roles for the components. The arrangement of proteins in the complex implies interdependence of their biochemical activities making it difficult to separate specific functions. We obtained purified human RAD50 and observed that it binds ATP, undergoes ATP-dependent conformational changes as well as having ATPase activity. Scanning force microscopy analysis clearly showed that RAD50 binds DNA although not as oligomers. RAD50 alone was not functional in tethering DNA molecules. ATP increased formation of RAD50 multimers which were however globular lacking extended coiled coils, in contrast to the MR complex where ATP induced oligomers have obvious coiled coils protruding from a central domain. These results suggest that MRE11 is important in maintaining the structural arrangement of RAD50 in the protein complex and perhaps has a role in reinforcing proper alignment of the coiled coils in the ATP-bound state. (C) 2015 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
Keyword:
MR(N) complex
Scanning force microscopy
Atomic force microscopy
DNA break repair
RAD50 purification
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3
论文数:
5.9K
被引数:
1.2W
机构
引用论文
DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP.png)
NUCLEIC ACIDS RESEARCH
OAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAI分享收藏Structure of the Rad50 DNA double-strand break repair protein in complex with DNA与DNA复合的Rad50 DNA双链断裂修复蛋白的结构EMBO JOURNALOAAI
EMBO JOURNALIF8.3
OAAI分享收藏分享收藏Compression Tests of ABS Specimens for UAV Components Produced via the FDM TechniqueTechnologiesOAAI
TechnologiesIF0
OAAI分享收藏
查看更多内容