.png){kind=icon}
返回
Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy
.png){kind=icon}
OA
AI
DOI：10.1364/OPTICA.4.000263.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
In fluorescence microscopy, the serial acquisition of two-dimensional images to form a three-dimensional (3D) volume limits the maximum imaging speed. This is particularly evident when imaging adherent cells in a light-sheet fluorescence microscopy format, as their elongated morphologies require similar to 200 image planes per image volume. Here, by illuminating the specimen with three light sheets, each independently detected, we present a light-efficient, cross-talk-free, and volumetrically parallelized 3D microscopy technique that is optimized for high-speed (up to 14 Hz) subcellular (300 nm lateral, 600 nm axial resolution) imaging of adherent cells. We demonstrate 3D imaging of intracellular processes, including cytoskeletal dynamics in single-cell migration and collective wound healing for 1500 and 1000 time points, respectively. Furthermore, we capture rapid biological processes, including the trafficking of early endosomes with velocities exceeding 10 mu m/s and calcium signaling in primary neurons. (C) 2017 Optical Society of America
Keyword:
FLUORESCENCE MICROSCOPY
CELL
ILLUMINATION
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
8.5
论文数:
2.4K
被引数:
2.1W
机构
引用论文
Integrating mechanism-based modeling with biomedical imaging to build practical digital twins for clinical oncology将基于机制的建模与生物医学成像集成，以构建用于临床肿瘤学的实用数字双胞胎
OAAI
OAAI分享收藏Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric Imaging of Adherent Cells.png)
BIOPHYSICAL JOURNALOAAI
BIOPHYSICAL JOURNALIF3.1
OAAI分享收藏分享收藏Advances in fluorescence labeling strategies for dynamic cellular imaging动态细胞成像荧光标记策略的研究进展NATURE CHEMICAL BIOLOGYOAAI
NATURE CHEMICAL BIOLOGYIF13.7
OAAI分享收藏Robust single-particle tracking in live-cell time-lapse sequences活细胞延时序列中的鲁棒单粒子跟踪NATURE METHODSOAAI
NATURE METHODSIF32.1
OAAI分享收藏分享收藏
查看更多内容