.png){kind=icon}
返回
ImFCS: A software for Imaging FCS data analysis and visualization
.png){kind=icon}
OA
AI
DOI：10.1364/OE.18.025468.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The multiplexing of fluorescence correlation spectroscopy (FCS), especially in imaging FCS using fast, sensitive array detectors, requires the handling of large amounts of data. One can easily collect in excess of 100,000 FCS curves a day, too many to be treated manually. Therefore, ImFCS, an open-source software which relies on standard image files was developed and provides a wide range of options for the calculation of spatial and temporal auto-and cross-correlations, as well as differences in Cross-Correlation Functions (Delta CCF). ImFCS permits fitting of standard models to correlation functions and provides optimized histograms of fitted parameters. Applications include the measurement of diffusion and flow with Imaging Total Internal Reflection FCS (ITIR-FCS) and Single Plane Illumination Microscopy FCS (SPIM-FCS) in biologically relevant samples. As a compromise between ITIR-FCS and SPIM-FCS, we extend the applications to Imaging Variable Angle-FCS (IVA-FCS) where sub-critical oblique illumination provides sample sectioning close to the cover slide. (C) 2007 Optical Society of America
Keyword:
INTERNAL-REFLECTION FLUORESCENCE
CORRELATION SPECTROSCOPY
CROSS-CORRELATION
DIFFUSION
MICROSCOPY
TRANSPORT
期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
机构
引用论文
Electron multiplying CCD based detection for spatially resolved fluorescence correlation spectroscopy.png)
OPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopyANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏Two beam cross correlation:: A method to characterize transport phenomena in micrometer-sized structuresANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏Highly inclined thin illumination enables clear single-molecule imaging in cells (vol 5, pg 159, 2008)NATURE METHODS
OAAI
NATURE METHODSIF32.1
OAAI分享收藏分享收藏
查看更多内容