.png){kind=icon}
返回
Interception Proximity Labeling for Interrogating Cell Efflux Microenvironment
.png){kind=icon}
PRE
AI
DOI：10.1021/acs.analchem.3c03879.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The difficulty in elucidating the microenvironment of extracellular H2O2 efflux has led to the lack of a critical extracellular link in studies of the mechanisms of redox signaling pathways. Herein, we mounted horseradish peroxidase (HRP) to glycans expressed globally on the living cell surface and constructed an interception proximity labeling (IPL) platform for H2O2 efflux. The release of endogenous H2O2 is used as a physiological switch for HRP to enable proximity labeling. Using this platform, we visualize the oxidative stress state of tumor cells under the condition of nutrient withdrawal, as well as that of macrophages exposed to nonparticulate stimuli. Furthermore, in combination with a proteomics technique, we identify candidate proteins at the invasion interface between fungal mimics (zymosan) and macrophages by interception labeling of locally accumulated H2O2 and confirm that Toll-like receptor 2 binds zymosan in a glycan-dependent manner. The IPL platform has great potential to elucidate the mechanisms underlying biological processes involving redox pathways.
Keyword:
HYDROGEN-PEROXIDE
HUMAN-NEUTROPHILS
PLASMA-MEMBRANE
BIOLOGY
VISUALIZATION
RECOGNITION
ACTIVATION
RECEPTORS
IMMUNITY
ZYMOSAN
期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
机构
引用论文
Performance analysis of energy-harvesting relay selection systems with multiple antennas in presence of transmit hardware impairments发射硬件损伤情况下多天线能量采集中继选择系统的性能分析
PREAI
分享收藏
The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors模式识别受体在先天免疫中的作用: Toll样受体的更新.png)
NATURE IMMUNOLOGY
OAAI
NATURE IMMUNOLOGYIF27.6
OAAI分享收藏
查看更多内容