Interferometrical single-molecule localization based on dynamic PSF engineering

doi:10.1364/OL.453113

2022-03-26

PRE

DOI：10.1364/OL.453113

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摘要

En 中文

We present a method for interferometric single-molecule localization based on dynamic point spread function (PSF) engineering. By using two galvo mirrors, a hexagonal PSF is constructed and the fluorescent signal under different illumination patterns could be acquired simultaneously. This method was evaluated using simulation, fluorescent nanosphere imaging, and single-molecule imaging. The study indicates a twofold improvement in localization precision while maintaining the same photon budget. This strategy, we believe, is a cost-effective way to increase the resolution of single-molecule localization microscopy. (C) 2022 Optica Publishing Group

Keyword:

MICROSCOPY

期刊

Optics Letters

IF:

3.3

论文数:

4.0W

被引数:

7.6W

机构

chinese academy of sciences

学者数:

56.7W

论文数: 45.0W

被引数: 704

guangxi university

学者数:

3.4W

论文数: 1.8W

被引数: 25

引用论文

Localization microscopy at doubled precision with patterned illumination 具有图案化照明的双倍精度的定位显微镜

NATURE METHODS

IF32.1

2019-12-09

138

OAAI

Cnossen, Jelmer; Hinsdale, Taylor; Thorsen, Rasmus O.; Siemons, Marijn; Schueder, Florian; Jungmann, Ralf; Smith, Carlas S.; Rieger, Bernd; Stallinga, Sjoerd

SIMPLE: Structured illumination based point localization estimator with enhanced precision 简单: 具有增强精度的基于结构化照明的点定位估计器

OPTICS EXPRESS

IF3.3

2019-08-14