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Light-sheet microscopy: a tutorial
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DOI：10.1364/AOP.10.000111.png)
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摘要
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This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted. (c) 2018 Optical Society of America
Keyword:
PLANE ILLUMINATION MICROSCOPY
FLUORESCENCE MICROSCOPY
BESSEL BEAM
STRUCTURED-ILLUMINATION
ADAPTIVE OPTICS
LONG-TERM
CORRELATION SPECTROSCOPY
STIMULATED-EMISSION
LATERAL RESOLUTION
DIFFRACTION LIMIT
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