Megapixel digital PCR

Kevin A. Heyries

doi:10.1038/NMETH.1640

2011-07-03

321

PRE

DOI：10.1038/NMETH.1640

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摘要

En 中文

We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.

Keyword:

POLYMERASE-CHAIN-REACTION

DNA

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期刊

Nature Methods

IF:

32.1

论文数:

7.3K

被引数:

12.7W

机构

University of British Columbia

学者数:

1.3K

论文数: 556

被引数: 0

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