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Microdevice DNA forensics by the simple tandem repeat method
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DOI：10.1016/j.chroma.2005.05.100.png)
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We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures. (c) 2005 Elsevier B.V. All rights reserved.
Keyword:
forensic DNA analysis
microdevice electrophoresis
microfluidics
short tandem repeat
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