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Multiphoton structured thin-plane imaging with a single optical path
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DOI：10.1364/OL.43.005271.png)
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摘要
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Optical sectioning has become an indispensable technique for high-speed volumetric imaging in the past decade. Here we present a novel optical-sectioning method that produces a thin plane of illumination by exploiting the spatial and temporal properties of multiphoton excitation. Critically, the illumination and detection share the same optical path, as in a conventional epi-fluorescence microscope configuration. Therefore, the imaged sample can be prepared as for standard fluorescence microscopy. Our method also leads to a laterally structured illumination pattern, and this feature can be utilized in structured illumination microscopy to further enhance the imaging performance. We show an example of such an approach, which achieves axial resolution finer than confocal microscopy. We also demonstrate the potential of the new method for biological applications by performing three-dimensional imaging of living Caenorhabditis elegans. (C) 2018 Optical Society of America
Keyword:
FLUORESCENCE MICROSCOPY
RESOLUTION
DEEP
期刊
IF:
3.3
论文数:
4.0W
被引数:
7.6W
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分享收藏Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms.png)
NATURE PHOTONICS
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NATURE PHOTONICSIF32.9
OAAI分享收藏Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination通过结构化照明在宽场荧光显微镜中提高三维分辨率BIOPHYSICAL JOURNALOAAI
BIOPHYSICAL JOURNALIF3.1
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