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Novel RNA base pair with higher specificity using single selenium atom
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DOI：10.1093/nar/gks010.png)
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摘要
En 中文
Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U in RNA (or T in DNA) pairs with G instead of A, might compromise the high specificity of the base pairing. The U/G wobble pairing is ubiquitous in RNA, especially in non-coding RNA. In order to increase U/A pairing specificity, we have hypothesized to discriminate against U/G wobble pair by tailoring the steric and electronic effects at the 2-exo position of uridine and replacing the 2-exo oxygen with a selenium atom. We report here the first synthesis of the 2-Se-U-RNAs as well as the 2-Se-uridine (U-Se) phosphoramidite. Our biophysical and structural studies of the U-Se-RNAs indicate that this single atom replacement can indeed create a novel U/A base pair with higher specificity than the natural one. We reveal that the U-Se/A pair maintains a structure virtually identical to the native U/A base pair, while discriminating against U/G wobble pair. This oxygen replacement with selenium offers a unique chemical strategy to enhance the base pairing specificity at the atomic level.
Keyword:
WOBBLE HYPOTHESIS
ESCHERICHIA-COLI
1ST POSITION
ANTICODON
ERRORS
NUCLEOSIDES
STABILITY
SOFTWARE
PROTEIN
CODONS
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期刊
IF:
13.1
论文数:
3.6W
被引数:
29.0W
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