Observations from the Proteomics Bench

Simone Koenig

doi:10.3390-proteomes12010006

2025-08-22

PRE

Simone Koenig *

Karin Schork

Martin Eisenacher

DOI：10.3390-proteomes12010006

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摘要

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许多蛋白质组学中的挑战源于实验的高通量特性。本文首先阐述了前分析阶段的问题，尽管组学领域的标准化呼吁已持续多年，这些问题依然存在。本文还基于在三种不同Orbitrap仪器上测量的三组参考数据，讨论了影响生物信息学分析的方面。尽管质谱技术和分析软件不断取得进展，但针对数据集的质量控制仍然是必要的，且基于诱饵的评估，尽管受到现代仪器的挑战，仍应加以利用。我们注意到，许多年轻研究人员将蛋白质组学视为一种成熟且易于应用的技术。然而，必须强调的是，该技术的最大潜力只有通过对其局限性的教育性处理才能得以实现。

Keyword:

proteomics

mass spectrometry

data quality control

bioinformatics

standardization

期刊

Proteomes

IF:

3.6

论文数:

413

被引数:

1.1K

机构

University of Münster

学者数:

832

论文数: 349

被引数: 1

ruhr-university bochum

学者数:

544

论文数: 210

被引数: 1

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