.png){kind=icon}
返回
Polarization-sensitive optics diffraction tomography
.png){kind=icon}
OA
AI
DOI：10.1364/OPTICA.415343.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor using 2D scattered fields measured for different illumination angles and polarizations. Conventional optical diffraction tomography (ODT) has been used as a quantitative, label-free 3D imaging method. It is based on the scalar formalism, which limits its application to isotropic samples. We achieve imaging of the birefringence of 3D objects through a reformulation of ODT based on vector diffraction theory. The off-diagonal components of the RI tensor reconstruction convey additional information that is not available in either conventional scalar ODT or 2D polarization microscopy. Finally, we show experimental reconstructions of 3D objects with a polarization-sensitive contrast metric quantitatively displaying the true birefringence of the samples. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Keyword:
PHASE MICROSCOPY
BIREFRINGENCE
HOLOGRAPHY
TISSUE
期刊
IF:
8.5
论文数:
2.4K
被引数:
2.1W
机构
引用论文
Reference-free polarization-sensitive quantitative phase imaging using single-point optical phase conjugation.png)
OPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏Rapid and Quantitative Imaging of Excitation Polarized Fluorescence Reveals Ordered Septin Dynamics in Live YeastBIOPHYSICAL JOURNAL
OAAI
BIOPHYSICAL JOURNALIF3.1
OAAI分享收藏Optical diffraction tomography for high resolution live cell imaging用于高分辨率活细胞成像的光学衍射层析成像OPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏分享收藏分享收藏Comparative study of iterative reconstruction algorithms for missing cone problems in optical diffraction tomographyOPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏
查看更多内容