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Protein-protein interactions
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DOI：10.1042/BST0380875.png)
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In the present article, we describe the two standard high-throughput methods for identification of protein complexes: two-hybrid screens and TAP (tandem affinity purification) tagging. These methods have been used to characterize the interactome of Saccharomyces cerevisiae, showing that the majority of proteins are part of complexes, and that complexes typically consist of a core to which are bound 'party' and 'dater' proteins. Complexes typically are merely the sum of their parts. A particularly interesting type of complex is the metabolon, containing enzymes within the same metabolic pathway. There is reasonably good evidence that metabolons exist, but they have not been detected using high-thoughput assays, possibly because of their fragility.
Keyword:
high-throughput method
interactome
metabolon
tandem affinity purification tag (TAP-tag)
two-hybrid screen
yeast
期刊
B
IF:
4.3
论文数:
7.8K
被引数:
1.4W
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Interaction proteomics: characterization of protein complexes using tandem affinity purification-mass spectrometry相互作用蛋白质组学: 使用串联亲和纯化-质谱法表征蛋白质复合物
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