Proteome sequencing goes deep

Alicia Richards

doi:10.1016/j.cbpa.2014.10.017

2015-02-01

Alicia Richards

Anna E. Merrill

Joshua J. Coon *

DOI：10.1016/j.cbpa.2014.10.017

原文链接

查看原文

摘要

En 中文

Advances in mass spectrometry (MS) have transformed the scope and impact of protein characterization efforts. Identifying hundreds of proteins from rather simple biological matrices, such as yeast, was a daunting task just a few decades ago. Now, expression of more than half of the estimated similar to 20 000 human protein coding genes can be confirmed in record time and from minute sample quantities. Access to proteomic information at such unprecedented depths has been fueled by strides in every stage of the shotgun proteomics workflow - from sample processing to data analysis - and promises to revolutionize our understanding of the causes and consequences of proteome variation.

Keyword:

TANDEM MASS-SPECTROMETRY

PEPTIDE IDENTIFICATION

HUMAN GENOME

PEAK-CAPACITY

DEPTH

QUANTIFICATION

ENCODE

DISSOCIATION

PERFORMANCE

DIGESTION

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Current Opinion in Chemical Biology

IF:

6.1

论文数:

3.1K

被引数:

1.1W

机构

university of wisconsin madison

学者数:

3.8W

论文数: 2.9W

被引数: 53

University of Wisconsin System

学者数:

6.7W

论文数: 5.8W

被引数: 382

引用论文

Rapid and Deep Human Proteome Analysis by Single-dimension Shotgun Proteomics

MOLECULAR & CELLULAR PROTEOMICS

IF5.5

2013-11-01

135

OAAI

Pirmoradian, Mohammad; Budamgunta, Harshavardhan; Chingin, Konstantin; Zhang, Bo; Astorga-Wells, Juan; Zubarev, Roman A.

Electrospun nanofibre bundles and yarns for tissue engineering applications: A review

Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine

IF0

2016-10-19

PREAI

Richard A O’Connor; Garrett B McGuinness

From the Human Genome to the Human Proteome 从人类基因组到人类蛋白质组

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

IF16.9

2014-07-30

PREAI

Munoz, Javier; Heck, Albert J. R.

GENCODE: The reference human genome annotation for The ENCODE Project GENCODE: ENCODE项目的参考人类基因组注释

GENOME RESEARCH

IF5.5

2012-09-05

3.9K

OAAI

Harrow, Jennifer; Frankish, Adam; Gonzalez, Jose M.; Tapanari, Electra; Diekhans, Mark; Kokocinski, Felix; Aken, Bronwen L.; Barrell, Daniel; Zadissa, Amonida; Searle, Stephen; Barnes, If; Bignell, Alexandra; Boychenko, Veronika; Hunt, Toby; Kay, Mike; Mukherjee, Gaurab; Rajan, Jeena; Despacio-Reyes, Gloria; Saunders, Gary; Steward, Charles; Harte, Rachel; Lin, Michael; Howald, Cedric; Tanzer, Andrea; Derrien, Thomas; Chrast, Jacqueline; Walters, Nathalie; Balasubramanian, Suganthi; Pei, Baikang; Tress, Michael; Manuel Rodriguez, Jose; Ezkurdia, Iakes; van Baren, Jeltje; Brent, Michael; Haussler, David; Kellis, Manolis; Valencia, Alfonso; Reymond, Alexandre; Gerstein, Mark; Guigo, Roderic; Hubbard, Tim J.

Electron Energy Loss Near Edge Structure (ELNES) on the Carbon K-Edge in Transition Metal Carbides with the Rock Salt Structure

Microscopy Microanalysis Microstructures

IF0

1995-01-01

OAAI

Alan J. Craven; Laurence A. J. Garvie

Comparative Proteomic Analysis of Eleven Common Cell Lines Reveals Ubiquitous but Varying Expression of Most Proteins

MOLECULAR & CELLULAR PROTEOMICS

IF5.5

2012-03-01

614

OAAI

Geiger, Tamar; Wehner, Anja; Schaab, Christoph; Cox, Juergen; Mann, Matthias

Ab initio calculations of polarizabilities and second hyperpolarizabilities in organic molecules with extended .pi.-electron conjugation

The Journal of Physical Chemistry

IF0

2002-05-01

PREAI

Pratibha Chopra; Louis Carlacci; Harry F. King; Paras N. Prasad

Proteomic and phosphoproteomic comparison of human ES and iPS cells

NATURE METHODS

IF32.1

2011-09-11

273

OAAI

Phanstiel, Douglas H.; Brumbaugh, Justin; Wenger, Craig D.; Tian, Shulan; Probasco, Mitchell D.; Bailey, Derek J.; Swaney, Danielle L.; Tervo, Mark A.; Bolin, Jennifer M.; Ruotti, Victor; Stewart, Ron; Thomson, James A.; Coon, Joshua J.

Effects of Column and Gradient Lengths on Peak Capacity and Peptide Identification in Nanoflow LC-MS/MS of Complex Proteomic Samples

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

IF2.7

2012-11-30

OAAI

Hsieh, Edward J.; Bereman, Michael S.; Durand, Stanley; Valaskovic, Gary A.; MacCoss, Michael J.

An in-situ spectroscopic study of the anodic oxide film on a cobalt electrode at pH 4–14 by potential-modulated reflectance 通过电位调制反射率对pH 4-14的钴电极上的阳极氧化膜进行原位光谱研究

Journal of Electroanalytical Chemistry and Interfacial Electrochemistry

IF0

1990-10-01

PREAI

Gerardo Larramona; Claudio Gutiérrez

查看更多内容