Quantitative super-resolution imaging with qPAINT

doi:10.1038/NMETH.3804

2016-03-28

318

Ralf Jungmann

Avendano, Maier S.

Mingjie Dai

Woehrstein, Johannes B.

DOI：10.1038/NMETH.3804

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摘要

En 中文

Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target.

Keyword:

NUCLEAR-PORE COMPLEX

SPATIAL-ORGANIZATION

RNA-POLYMERASE

DNA-PAINT

MICROSCOPY

MOLECULES

PROBES

FLUORESCENCE

BRUCHPILOT

MATURATION

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期刊

Nature Methods

IF:

32.1

论文数:

7.2K

被引数:

12.7W

机构

Harvard University

学者数:

26.5W

论文数: 22.0W

被引数: 28.7W

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