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Raman spectroscopy-based DNA damage profile induced by UVB, UVC, and gamma-ray irradiation under equivalent energy absorption conditions
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DOI：10.1088/1402-4896/ae648b.png)
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Abstract
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Linear energy transfer (LET) is a physical parameter commonly used as an indicator of radiation quality in radiation therapy. However, the molecular damage profile derived from Raman spectroscopy may provide a more detailed and sensitive measure of radiation quality than LET alone. Raman spectroscopy was used to characterize DNA damage induced by UVB, UVC, and gamma-ray irradiation under equivalent energy absorption conditions. A total of 89 Raman bands were identified in non-irradiated DNA, among which 79 displayed intensity or positional changes after irradiation. We introduced a 'damage profile,' which summarizes changes in Raman intensity and band position to characterize the molecular state of DNA. The overall intensity-based damage profiles showed similar tendencies among the three radiation types. In contrast, shift-based profiles revealed distinct patterns: UVB and UVC were comparable, whereas gamma-rays showed clearly different behavior, consistent with their distinct damage mechanisms. UV irradiation produced pronounced perturbations in both pyrimidine-related bands and backbone-associated vibrations, reflecting pyrimidine dimer formation and local conformational changes. Gamma-ray irradiation caused more localized but characteristic modifications, particularly in guanine-associated bands indicative of oxidative lesions. These results demonstrate that Raman-based DNA damage profiles provide sensitive molecular fingerprints capable of distinguishing radiation quality and may contribute to improved prediction of biological effectiveness in radiation therapy.
Keywords:
Raman spectroscopy
DNA damage
UVB
UVC
gamma-ray
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