Rapid nonlinear image scanning microscopy

Ingo Gregor

doi:10.1038/NMETH.4467

2017-10-16

PRE

DOI：10.1038/NMETH.4467

原文链接

原文求助

摘要

En 中文

Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.

Keyword:

STRUCTURED-ILLUMINATION MICROSCOPY

INTACT MOUSE-BRAIN

2ND-HARMONIC GENERATION

CONFOCAL MICROSCOPY

FLUORESCENCE MICROSCOPY

LIVE CELLS

RESOLUTION

COLLAGEN

REASSIGNMENT

IMPROVES

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Nature Methods

IF:

32.1

论文数:

7.2K

被引数:

12.7W

机构

Arizona State University

学者数:

2.7W

论文数: 2.5W

被引数: 4.2W

university gottingen hospital

学者数:

4.8K

论文数: 3.5K

被引数: 4

University of Gottingen

学者数:

2.5W

论文数: 2.1W

被引数: 36

查看更多机构

引用论文

Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution 非线性结构照明显微镜: 理论上无限分辨率的宽场荧光成像

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

IF9.1

2005-09-02

2.2K

OAAI

Gustafsson, MGL

Survey of Plagiarism Detection Approaches and Big data Techniques related to Plagiarism Candidate Retrieval

Proceedings of the 2nd international Conference on Big Data, Cloud and Applications

IF0

2017-03-29

PREAI

Oumaima Hourrane; El Habib Benlahmar

High-resolution confocal Raman microscopy using pixel reassignment

OPTICS LETTERS

IF3.3

2016-08-09

PREAI

Roider, Clemens; Ritsch-Marte, Monika; Jesacher, Alexander

Superconcentration of light: circumventing the classical limit to achievable irradiance 光的过度集中: 绕过可达到的辐照度的经典极限

OPTICS LETTERS

IF3.3

2016-04-29

PREAI

Roth, Stephan; Sheppard, Colin J. R.; Heintzmann, Rainer

Macromolecular-scale resolution in biological fluorescence microscopy

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

IF9.1

2006-08-01

494

OAAI

Donnert, Gerald; Keller, Jan; Medda, Rebecca; Andrei, M. Alexandra; Rizzoli, Silvio O.; Luehrmann, Reinhard; Jahn, Reinhard; Eggeling, Christian; Hell, Stefan W.

Correlating confocal microscopy and atomic force indentation reveals metastatic cancer cells stiffen during invasion into collagen I matrices

SCIENTIFIC REPORTS

IF3.9

2016-01-27

135

OAAI

Staunton, Jack R.; Doss, Bryant L.; Lindsay, Stuart; Ros, Robert

In vivo three-photon microscopy of subcortical structures within an intact mouse brain 完整小鼠大脑皮质下结构的体内三光子显微镜

NATURE PHOTONICS

IF32.9

2013-01-20

1.3K