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Real-time high dynamic range laser scanning microscopy
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DOI：10.1038/ncomms11077.png)
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摘要
En 中文
In conventional confocal/multiphoton fluorescence microscopy, images are typically acquired under ideal settings and after extensive optimization of parameters for a given structure or feature, often resulting in information loss from other image attributes. To overcome the problem of selective data display, we developed a new method that extends the imaging dynamic range in optical microscopy and improves the signal-to-noise ratio. Here we demonstrate how real-time and sequential high dynamic range microscopy facilitates automated three-dimensional neural segmentation. We address reconstruction and segmentation performance on samples with different size, anatomy and complexity. Finally, in vivo real-time high dynamic range imaging is also demonstrated, making the technique particularly relevant for longitudinal imaging in the presence of physiological motion and/or for quantification of in vivo fast tracer kinetics during functional imaging.
Keyword:
SINGLE-CELL
HIGH-THROUGHPUT
SENSITIVITY
SCALE
DYES
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期刊
IF:
15.7
论文数:
9.3W
被引数:
91.2W
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