Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction

Matthew Wilding

doi:10.1039/c7gc02343j

2017-01-01

Matthew Wilding *

Thomas S. Peat

Subha Kalyaanamoorthy

Janet Newman

Colin Scott

Lars S. Jermiin

DOI：10.1039/c7gc02343j

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摘要

En 中文

The development of new biocatalysts using ancestral sequence reconstruction is reported. When applied to an omega-transaminase, the ancestral proteins demonstrated novel and superior activities with eighty percent of the forty compounds tested compared to the modern day protein, and improvements in activity of up to twenty fold. These included a range of compounds pertinent as feedstocks in polyamide manufacture.

Keyword:

3-ISOPROPYLMALATE DEHYDROGENASE

OMEGA-TRANSAMINASE

DIRECTED EVOLUTION

ESCHERICHIA-COLI

ACID

PROTEINS

IDENTIFICATION

CHEMISTRY

PATHWAY

ENZYMES

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Green Chemistry

IF:

9.2

论文数:

1.3W

被引数:

7.6W

机构

commonwealth scientific & industrial research organisation (csiro)

学者数:

2.3W

论文数: 2.3W

被引数: 20

引用论文

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