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Shotgun glycomics: a microarray strategy for functional glycomics
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DOI：10.1038/NMETH.1540.png)
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摘要
En 中文
Major challenges of glycomics are to characterize a glycome and identify functional glycans as ligands for glycan-binding proteins (GBPs). To address these issues we developed a general strategy termed shotgun glycomics. We focus on glycosphingolipids (GSLs), a class of glycoconjugates that is challenging to study, recognized by toxins, antibodies and GBPs. We derivatized GSLs extracted from cells with a heterobifunctional fluorescent tag suitable for covalent immobilization. We separated fluorescent GSLs by multidimensional chromatography, quantified them and coupled them to glass slides to create GSL shotgun microarrays. Then we interrogated the microarrays with cholera toxin, antibodies and sera from individuals with Lyme disease to identify biologically relevant GSLs that we subsequently characterized by mass spectrometry. Shotgun glycomics incorporating GSLs and potentially glycoprotein-derived glycans is an approach for accessing the complex glycomes of animal cells and is a strategy for focusing structural analyses on functionally important glycans.
Keyword:
PERFORMANCE LIQUID-CHROMATOGRAPHY
GANGLIOSIDE LACTONES
GLYCOSPHINGOLIPIDS
ANTIBODIES
BACTERIA
ANTIGEN
SYSTEM
BRAIN
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32.1
论文数:
7.3K
被引数:
12.7W
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Fluorescent Glycosylamides Produced by Microscale Derivatization of Free Glycans for Natural Glycan Microarrays.png)
ACS CHEMICAL BIOLOGYOAAI
ACS CHEMICAL BIOLOGYIF3.8
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