.png){kind=icon}
返回
Simplified ChIP-exo assays
.png){kind=icon}
OA
AI
DOI：10.1038/s41467-018-05265-7.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
ChIP-seq and ChIP-exo identify where proteins bind along any genome in vivo. Although ChIP-seq is widely adopted in academic research, it has inherently high noise. In contrast, ChIP-exo has relatively low noise and achieves near-base pair resolution. Consequently, and unlike other genomic assays, ChIP-exo provides structural information on genome-wide binding proteins. Construction of ChIP-exo libraries is technically difficult. Here we describe greatly simplified ChIP-exo methods, each with use-specific advantages. This is achieved through assay optimization and use of Tn5 tagmentation and/or single-stranded DNA ligation. Greater library yields, lower processing time, and lower costs are achieved. In comparing assays, we reveal substantial limitations in other ChIP-based assays. Importantly, the new ChIP-exo assays allow high-resolution detection of some protein-DNA interactions in organs and in as few as 27,000 cells. It is suitable for high-throughput parallelization. The simplicity of ChIP-exo now makes it a highly appropriate substitute for ChIP-seq, and for broader adoption.
Keyword:
DNA
INVIVO
TN5
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
15.7
论文数:
9.4W
被引数:
91.2W
机构
引用论文
Development of an Illumina-based ChIP-exonuclease method provides insight into FoxA1-DNA binding properties.png)
GENOME BIOLOGYOAAI
GENOME BIOLOGYIF9.4
OAAI分享收藏Cation ordering and structural variations with temperature in MgAl2O4spinel: An X-ray single-crystal studyMgAl2O4 尖晶石中阳离子有序性和结构随温度的变化: x射线单晶研究
PREAI
分享收藏
Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition通过高密度体外转座快速，低投入，低偏倚构建shot弹枪片段库GENOME BIOLOGYOAAI
GENOME BIOLOGYIF9.4
OAAI分享收藏Long-term efficacy and safety of rituximab in IgG4-related disease: Data from a French nationwide study of thirty-three patients利妥昔单抗治疗IgG4-related疾病的长期疗效和安全性: 来自法国全国33例患者的研究数据PLOS ONEOAAI
PLOS ONEIF0
OAAI分享收藏Single-stranded DNA library preparation for the sequencing of ancient or damaged DNANATURE PROTOCOLS
NATURE PROTOCOLSIF16
PREAI
分享收藏
查看更多内容