Simultaneous multiplane in vivo nonlinear microscopy using spectral encoding

Lauren E. Grosberg

doi:10.1364/OL.37.002967

2012-07-13

Lauren E. Grosberg *

Brenda R. Chen

Elizabeth M. C. Hillman

DOI：10.1364/OL.37.002967

原文链接

查看原文

摘要

En 中文

Conventional point-by-point imaging schemes for laser scanning microscopy limit acquisition speeds, particularly when imaging three-dimensional volumes. We report a novel approach that achieves parallelization of multiple fields of view through the use of spectral encoding. By focusing two or more beams of different wavelengths at different positions within a suitable tissue, fluorescence or second/third harmonic generation emissions from these regions can be uniquely separated. We demonstrate that this approach can allow simultaneous in vivo imaging of fluorescence in two planes within the living rodent cortex, and of second harmonic generation in fresh tissue. (C) 2012 Optical Society of America

Keyword:

MULTIPHOTON MICROSCOPY

2-PHOTON MICROSCOPY

DYNAMICS

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Optics Letters

IF:

3.3

论文数:

4.0W

被引数:

7.6W

机构

Columbia University

学者数:

7.1W

论文数: 6.4W

被引数: 263

引用论文

Erratum: Bonus of rebate?: The impact of income framing on spending and saving

Journal of Behavioral Decision Making

IF0

2006-09-07

PREAI

Nicholas Epley; Dennis Mak; Lorraine Chen Idson

Spontaneous fluctuations in brain activity observed with functional magnetic resonance imaging 通过功能磁共振成像观察到的大脑活动的自发波动

NATURE REVIEWS NEUROSCIENCE

IF26.7

2007-09-01

5.7K

PREAI

Fox, Michael D.; Raichle, Marcus E.

A technical review on gas diffusion, mechanism and medium of PEM fuel cell PEM燃料电池气体扩散、机理及介质技术综述

Ionics

IF0

2014-11-30

PREAI

Arunkumar Jayakumar; Sundar Pethaiah Sethu; Maximiano Ramos; John Robertson; Ahmed Al-Jumaily

Encouraging Compliance: Bonuses Versus Fines in Inspection Games

Journal of Law, Economics, and Organization

IF0

2013-02-14

PREAI

D. Nosenzo; T. Offerman; M. Sefton; A. van der Veen

British Society of Gastroenterology guidelines on the diagnosis and management of Barrett's oesophagus

Gut

IF0

2013-10-28

OAAI

Rebecca C Fitzgerald; Massimiliano di Pietro; Krish Ragunath; Yeng Ang; Jin-Yong Kang; Peter Watson; Nigel Trudgill; Praful Patel; Philip V Kaye; Scott Sanders; Maria O'Donovan; Elizabeth Bird-Lieberman; Pradeep Bhandari; Janusz A Jankowski; Stephen Attwood; Simon L Parsons; Duncan Loft; Jesper Lagergren; Paul Moayyedi; Georgios Lyratzopoulos; John de Caestecker

Cortical depth-specific microvascular dilation underlies laminar differences in blood oxygenation level-dependent functional MRI signal

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

IF9.1

2010-08-09

258

OAAI

Tian, Peifang; Teng, Ivan C.; May, Larry D.; Kurz, Ronald; Lu, Kun; Scadeng, Miriam; Hillman, Elizabeth M. C.; De Crespigny, Alex J.; D'Arceuil, Helen E.; Mandeville, Joseph B.; Marota, John J. A.; Rosen, Bruce R.; Liu, Thomas T.; Boas, David A.; Buxton, Richard B.; Dale, Anders M.; Devor, Anna

Imaging cellular network dynamics in three dimensions using fast 3D laser scanning

NATURE METHODS

IF32.1

2006-12-03

350

PREAI

Goebel, Werner; Kampa, Bjoern M.; Helmchen, Fritjof

Optimal Separable Algorithms to Compute the Reverse Euclidean Distance Transformation and Discrete Medial Axis in Arbitrary Dimension

IEEE Transactions on Pattern Analysis and Machine Intelligence

IF0

2007-03-01