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Single-cell analysis and sorting using droplet-based microfluidics
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DOI：10.1038/nprot.2013.046.png)
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摘要
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We present a droplet-based microfluidics protocol for high-throughput analysis and sorting of single cells. Compartmentalization of single cells in droplets enables the analysis of proteins released from or secreted by cells, thereby overcoming one of the major limitations of traditional flow cytometry and fluorescence-activated cell sorting. As an example of this approach, we detail a binding assay for detecting antibodies secreted from single mouse hybridoma cells. Secreted antibodies are detected after only 15 min by co-compartmentalizing single mouse hybridoma cells, a fluorescent probe and single beads coated with anti-mouse IgG antibodies in 50-pl droplets. The beads capture the secreted antibodies and, when the captured antibodies bind to the probe, the fluorescence becomes localized on the beads, generating a clearly distinguishable fluorescence signal that enables droplet sorting at similar to 200 Hz as well as cell enrichment. The microfluidic system described is easily adapted for screening other intracellular, cell-surface or secreted proteins and for quantifying catalytic or regulatory activities. In order to screen similar to 1 million cells, the microfluidic operations require 2-6 h; the entire process, including preparation of microfluidic devices and mammalian cells, requires 5-7 d.
Keyword:
POLYMERASE-CHAIN-REACTION
HIGH-THROUGHPUT ANALYSIS
REAL-TIME
PICOLITER-VOLUME
ON-CHIP
ENCAPSULATION
ASSAYS
FLOW
AMPLIFICATION
SURFACTANTS
期刊
IF:
16
论文数:
4.0K
被引数:
5.6W
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