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Smartphone Fluorescence Spectroscopy
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DOI：10.1021/ac502080t.png)
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摘要
En 中文
We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays. We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of specific nucleic acid sequences in a liquid test sample and compared performance against a conventional laboratory fluorimeter. The capability of distinguishing a one-point mismatch is also demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route toward portable biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.
Keyword:
RESONANCE ENERGY-TRANSFER
DNA
GENOMICS
MIR-21
NANOPARTICLES
HYBRIDIZATION
MICRORNA-21
ASSAYS
GENES
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期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
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