.png){kind=icon}
返回
Specific sphingolipid content decrease in Cerkl knockdown mouse retinas
.png){kind=icon}
OA
AI
DOI：10.1016/j.exer.2013.03.003.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Sphingolipids (SPLs) are finely tuned structural compounds and bioactive molecules involved in membrane fluidity and cellular homeostasis. The core sphingolipid, ceramide (CER), and its derivatives, regulate several crucial processes in neuronal cells, among them cell differentiation, cell cell interactions, membrane conductance, synaptic transmission, and apoptosis. Mutations in Ceramide Kinase-Like (CERKL) cause autosomal recessive Retinitis Pigmentosa and Cone Rod Dystrophy. The presence of a conserved lipid kinase domain and the overall similarity with CERK suggested that CERKL might play a role in the SPL metabolism as a CER kinase. Unfortunately, CERKL function and substrate(s), as well as its contribution to the retinal etiopathology, remain as yet unknown. In this work we aimed to characterize the mouse retinal sphingolipidome by UPLC-TOF to first, thoroughly investigate the SPL composition of the murine retina, compare it to our Cerkl -/- model, and finally assess new possible CERKL substrates by phosphorus quantification and protein-lipid overlay. Our results showed a consistent and notable decrease of the retinal SPL content (mainly ranging from 30% to 60%) in the Cerkl -/- compared to WT retinas, which was particularly evident in the glucosyl/galactosyl ceramide species (Glc/GalCer) whereas the phospholipids and neutral lipids remained unaltered. Moreover, evidence in favor of CERKL binding to GlcCer, GalCer and sphingomyelin has been gathered. Altogether, these results highlight the involvement of CERKL in the SPL metabolism, question its role as a kinase, and open new scenarios concerning its function. (C) 2013 Elsevier Ltd. All rights reserved.
Keyword:
knockout models
retinal dystrophy
sphingolipid
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
2.7
论文数:
7.7K
被引数:
1.6W
机构
引用论文
Mutations in rpoD that increase expression of genes in the mal regulon of Escherichia coli K-12
PREAI
分享收藏Diabetes alters sphingolipid metabolism in the retina - A potential mechanism of cell death in diabetic retinopathy.png)
DIABETESOAAI
DIABETESIF7.5
OAAI分享收藏Mutation of CERKL, a novel human ceramide kinase gene, causes autosomal recessive retinitis pigmentosa (RP26)一种新的人类神经酰胺激酶基因CERKL的突变导致常染色体隐性视网膜色素变性 (RP26)OAAI
OAAI分享收藏
查看更多内容