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Statistical comparative study between the conventional DPPH• spectrophotometric and dropping DPPH• analytical method without spectrophotometer: Evaluation for the advancement of antioxidant activity analysis
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A
DOI:10.1016/j.foodchem.2019.02.110.png)
Abstract
En 中文
Recently, new approaches for measuring antioxidant activity have been developed to eliminate or reduce the use of a spectrophotometer. All analytical methods must provide consistent and reliable results, which should be compared with statistical models. Therefore, we compared the conventional and dropping DPPH center dot methods with three widely used standards. We employed and compared with three statistical methods. Good R-squared (R-2= >= 0.96) confirmed the similarity in comparison of using dropping DPPH center dot method compared with the conventional DPPH center dot method and reported lower deviations (ARD= -0.32 to 0.13; RMSD= 0.10 to 1.15). Bland-Altman method (95% CI) demonstrated a good agreement between the two methods using standards and grape extracts (skin and seed). These findings suggested that the dropping DPPH center dot method proved cheaper (without spectrophotometer) and correlated well with the conventional DPPH center dot method. Therefore, this method could be affordably conducted in research laboratories in developing and less developed countries.
Keywords:
Conventional DPPH center dot method
Dropping DPPH center dot method
Antioxidant activity
Statistical studies
Bland-Altman method
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IF:
9.8
Papers:
4.6W
Citations:
24.4W
