.png){kind=icon}
返回
STED microscopy with a supercontinuum laser source
.png){kind=icon}
OA
AI
DOI：10.1364/OE.16.009614.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate preparations of laser pulses and conveniently provides multicolor imaging. Operating at pulse repetition rates around 1 MHz, it also affords reduced photobleaching rates by allowing the fluorophore to relax from excitable metastable dark states involved in photodegradation. The imaging of dense nanoparticles and of the microtubular network of mammalian cells evidences a spatial resolution of 30-50 nm in the focal plane, i.e. by a factor of 8-9 beyond the diffraction barrier. (c) 2008 Optical Society of America.
Keyword:
FLUORESCENCE MICROSCOPY
STIMULATED-EMISSION
RESOLUTION
FIBERS
LIMIT
期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
机构
引用论文
Model estimates of CO2 emissions from soil in response to global warming响应全球变暖的土壤CO2排放模型估计.png)
Nature
NatureIF0
PREAI
分享收藏Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)通过随机光学重建显微镜 (STORM) 进行亚衍射极限成像NATURE METHODS
OAAI
NATURE METHODSIF32.1
OAAI分享收藏Visible continuum generation in air-silica microstructure optical fibers with anomalous dispersion at 800 nmOPTICS LETTERS
OPTICS LETTERSIF3.3
PREAI
分享收藏Ultra-high resolution imaging by fluorescence photoactivation localization microscopy荧光光活化定位显微镜的超高分辨率成像BIOPHYSICAL JOURNALOAAI
BIOPHYSICAL JOURNALIF3.1
OAAI分享收藏分享收藏
查看更多内容