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Super-Resolution Fluorescence Microscopy

delete2009-06-01
delete1.6K
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B
Bo Huang *
M
Mark Bates
X
Xiaowei Zhuang
DOI:10.1146/annurev.biochem.77.061906.092014delete
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摘要

摘要

En 中文
Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.
Keyword:
diffraction limit
fluorescence imaging
fluorescent protein
live-cell imaging
photoswitchable dye
single molecule
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期刊

Annual Review of Biochemistry 封面图
Annual Review of Biochemistry
IF:
20.5
论文数:
2.2K
被引数:
2.1W

机构

H
Harvard University
学者数:
26.5W
论文数: 22.0W
被引数: 28.7W
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