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Super-resolution microscopy for nanosensing
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DOI：10.1002/wnan.130.png)
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摘要
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The recent advances in optical microscopy enable the simultaneous visualization of thousands of structural and signaling molecules as they dynamically rearrange within living cells. Super-resolution microscopy offers an unprecedented opportunity to define the molecular mechanisms of nanosensing through direct observation of protein movement. This technology provides a real-time readout of how genetically targeted molecular perturbations affect protein interactions. As we strive to meet the challenge offered by the opportunity to ask questions about the mechanism of cell that we never thought we could answer, we need to be aware that the new technologies are still evolving. The current limitations of each technique need to be considered when matching them to specific biological questions. In this review, we briefly describe the principles of super-resolution optical microscopy and focus on comparing the characteristics of each technique that are important for their use in studying nanosensing in the cellular microenvironment. (C) 2011 John Wiley & Sons, Inc. WIREs Nanomed Nanobiotechnol 2011 3 247-255 DOI:10.1002/wnan.130
Keyword:
STRUCTURED-ILLUMINATION MICROSCOPY
PHOTOACTIVATED LOCALIZATION MICROSCOPY
OPTICAL RECONSTRUCTION MICROSCOPY
FLUORESCENCE MICROSCOPY
STIMULATED-EMISSION
LIVING CELLS
CAULOBACTER-CRESCENTUS
DIFFRACTION-LIMIT
RESOLUTION LIMIT
STED MICROSCOPY
期刊
IF:
8.2
论文数:
924
被引数:
5.7K
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