Super-resolution optofluidic scanning microscopy

Biagio Mandracchia

doi:10.1039/d0lc00889c

2021-01-01

Biagio Mandracchia

Jeonghwan Son

Shu Jia *

DOI：10.1039/d0lc00889c

原文链接

查看原文

摘要

En 中文

Optofluidics enables visualizing diverse anatomical and functional traits of single-cell specimens with new degrees of imaging capabilities. However, the current optofluidic microscopy systems suffer from either low resolution to reveal subcellular details or incompatibility with general microfluidic devices or operations. Here, we report optofluidic scanning microscopy (OSM) for super-resolution, live-cell imaging. The system exploits multi-focal excitation using the innate fluidic motion of the specimens, allowing for minimal instrumental complexity and full compatibility with various microfluidic configurations. The results present effective resolution doubling, optical sectioning and contrast enhancement. We anticipate the OSM system to offer a promising super-resolution optofluidic paradigm for miniaturization and different levels of integration at the chip scale.

Keyword:

SHEET FLUORESCENCE MICROSCOPY

CELLS

MICROFLUIDICS

RESOLUTION

LIVE

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Lab on a Chip

IF:

5.4

论文数:

9.0K

被引数:

3.3W

机构

university system of georgia

学者数:

7.3W

论文数: 6.6W

被引数: 101

引用论文

Fucose supplementation in leukocyte adhesion deficiency type II

Blood

IF0

2000-06-01

PREAI

Amos Etzioni; Michela Tonetti

A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM 用于图像扫描显微镜的强大且多功能的平台，可实现超分辨率FLIM

NATURE METHODS

IF32.1

2019-01-14

132

OAAI

Castello, Marco; Tortarolo, Giorgio; Buttafava, Mauro; Deguchi, Takahiro; Villa, Federica; Koho, Sami; Pesce, Luca; Oneto, Michele; Pelicci, Simone; Lanzano, Luca; Bianchini, Paolo; Sheppard, Colin J. R.; Diaspro, Alberto; Tosi, Alberto; Vicidomini, Giuseppe

Optofluidics in bio-imaging applications

PHOTONICS RESEARCH

IF7.2

2019-04-17

PREAI

Chen, Sihui; Hao, Rui; Zhang, Yi; Yang, Hui

Ultrafast confocal fluorescence microscopy beyond the fluorescence lifetime limit

OPTICA

IF8.5

2018-01-29

104

OAAI

Mikami, Hideharu; Harmon, Jeffrey; Kobayashi, Hirofumi; Hamad, Syed; Wang, Yisen; Iwata, Osamu; Suzuki, Kengo; Ito, Takuro; Aisaka, Yuri; Kutsuna, Natsumaro; Nagasawa, Kazumichi; Watarai, Hiroshi; Ozeki, Yasuyuki; Goda, Keisuke

3D hydrodynamic focusing microfluidics for emerging sensing technologies

BIOSENSORS & BIOELECTRONICS

IF10.5

2015-05-01

PREAI

Daniele, Michael A.; Boyd, Darryl A.; Mott, David R.; Ligler, Frances S.

JAPANESE PERSPECTIVE ON REDUCTION IN LIFESTYLE DISEASE RISK IN IMMIGRANT JAPANESE BRAZILIANS: A DOUBLE‐BLIND, PLACEBO‐CONTROLLED INTERVENTION STUDY ON PALATINOSE

Clinical and Experimental Pharmacology and Physiology

IF0

2007-10-30

PREAI

Y Yamori; M Mori; H Mori; J Kashimura; T Sakuma; PM Ishikawa; E Moriguchi; Y Moriguchi

Intelligent image-based deformation-assisted cell sorting with molecular specificity

NATURE METHODS

IF32.1

2020-05-25

113

OAAI

Nawaz, Ahmad Ahsan; Urbanska, Marta; Herbig, Maik; Noetzel, Martin; Kraeter, Martin; Rosendahl, Philipp; Herold, Christoph; Toepfner, Nicole; Kubankova, Marketa; Goswami, Ruchi; Abuhattum, Shada; Reichel, Felix; Mueller, Paul; Taubenberger, Anna; Girardo, Salvatore; Jacobi, Angela; Guck, Jochen

Virtual-freezing fluorescence imaging flow cytometry

NATURE COMMUNICATIONS

IF15.7

2020-03-06

103

OAAI

Mikami, Hideharu; Kawaguchi, Makoto; Huang, Chun-Jung; Matsumura, Hiroki; Sugimura, Takeaki; Huang, Kangrui; Lei, Cheng; Ueno, Shunnosuke; Miura, Taichi; Ito, Takuro; Nagasawa, Kazumichi; Maeno, Takanori; Watarai, Hiroshi; Yamagishi, Mai; Uemura, Sotaro; Ohnuki, Shinsuke; Ohya, Yoshikazu; Kurokawa, Hiromi; Matsusaka, Satoshi; Sun, Chia-Wei; Ozeki, Yasuyuki; Goda, Keisuke

High-throughput microfluidic imaging flow cytometry 高通量微流控成像流式细胞术

CURRENT OPINION IN BIOTECHNOLOGY

IF7

2019-02-01

107

PREAI

Stavrakis, Stavros; Holzner, Gregor; Choo, Jaebum; DeMello, Andrew

Automating multimodal microscopy with NanoJ-Fluidics

NATURE COMMUNICATIONS

IF15.7

2019-03-15

OAAI

Almada, Pedro; Pereira, Pedro M.; Cuiley, Sian; Caillol, Ghislaine; Boroni-Rueda, Fanny; Dix, Christina L.; Charras, Guillaume; Baum, Buzz; Laine, Romain F.; Leterrier, Christophe; Henriques, Ricardo

查看更多内容