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SYNPLA, a method to identify synapses displaying plasticity after learning
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DOI：10.1073/pnas.1919911117.png)
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Which neural circuits undergo synaptic changes when an animal learns? Although it is widely accepted that changes in synaptic strength underlie many forms of learning and memory, it remains challenging to connect changes in synaptic strength at specific neural pathways to specific behaviors and memories. Here we introduce SYNPLA (synaptic proximity ligation assay), a synapse-specific, high-throughput, and potentially brain-wide method capable of detecting circuit-specific learning-induced synaptic plasticity.
Keyword:
proximity ligation assay
synaptic potentiation
fear conditioning
defense conditioning
GluA1
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10.8W
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73.5W
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