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TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy
DOI:10.1038/s41598-017-01122-7.png)
摘要
En 中文
Optimization of sample, imaging and data processing parameters is an essential task in localization based super-resolution microscopy, where the final image quality strongly depends on the imaging of single isolated fluorescent molecules. A computational solution that uses a simulator software for the generation of test data stacks was proposed, developed and tested. The implemented advanced physical models such as scalar and vector based point spread functions, polarization sensitive detection, drift, spectral crosstalk, structured background etc., made the simulation results more realistic and helped us interpret the final super-resolved images and distinguish between real structures and imaging artefacts.
Keyword:
RESOLUTION LIMIT
FLUOROPHORES
GUIDE
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期刊
IF:
3.9
论文数:
28.3W
被引数:
83.5W
机构
引用论文
Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging
NATURE METHODS
IF32.1
Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
NATURE METHODS
IF32.1
Rotational Mobility of Single Molecules Affects Localization Accuracy in Super-Resolution Fluorescence Microscopy
NANO LETTERS
IF9.1
Simultaneous measurement of position and color of single fluorescent emitters using diffractive optics
OPTICS LETTERS
IF3.3

